The apolipoprotein E gene plays an important role in the pathogenesis of Alzheimer's disease , and amyloid plaque comprised mostly of the amyloid-beta peptide ) is one of the major hallmarks of AD. However, the relationship between these two important molecules is poorly understood. We examined how A treatment affects APOE expression in cultured cells and tested the role of the transcription factor NF-B in APOE gene regulation. To delineate NF-B's role, we have characterized a 1098 nucleotide segment containing the (...) 5'-flanking region of the human APOE gene . Sequence analysis of this region suggests the presence of two potential NF-B elements. To demonstrate promoter activity, the region was cloned upstream of a promoterless luciferase gene. This segment was able to drive expression of luciferase in transient transfections of human fetal glial cells. Promoter activity was stimulated twofold by A treatment. Pretreatment with double-stranded DNA decoy oligonucleotides against NF-B reduced A stimulation. Deletion and mutagenetic analyses demonstrated that the distal NF-B element was functional and showed a strong DNA-protein complex band in gel shift analysis, similar to that from control NF-B consensus element. An anti-inflammatory and anti-NF-B drug, sodium salicylate, significantly blocked A-induced APOE promoter function. Our data provide evidence that upregulation of APOE by A in astroglial cells is mediated by an NF-B-element present in the 5'-flanking region of the APOE gene. (shrink)

Abstract. There is an ongoing debate in the philosophical and jurisprudential literature regarding the nature and possibility of Contract theory. On one hand, are those who argue (or assume) that there is, or should be, a single, general, universal theory of Contract Law, one applicable to all jurisdictions and all times. On the other hand, are those who assert that Contract theory should be localized to particular times and places, perhaps even with different theories for different types of agreements. This (...) article considers one facet of this debate: evaluating the relevance of the fact that the remedies available for breach of contract can vary significantly from one jurisdiction to another. This wide variation in remedies for breach of a (contractual) promise is one central difference between promises in morality and enforceable agreements in law. The article asserts that variation of remedies strongly supports the conclusion that there is (and can be) no general, universal theory of Contract Law. (shrink)

Twenty essays based on papers delivered at the International Symposium on the Varieties of Marxism held at the VanLeer Jerusalem Foundation 16-19 June 1974 and dedicated to the memory of George Lichtheim, an eminent historian of Marxism. The overall message is that, far from being a monolithic, crystal clear explanation of the universe, Marxism continues to be a multi-faceted ideology with diverse expressions in diverse cultures, spawning a wide spectrum of "official" prophets, each sincerely convinced that he or she is (...) correct and all others are wrong. Kamenka in his contribution, while somewhat too easily chiding Marx for being too absolute in his prescriptive ethics, suggests that the varieties of Marxism stem from "ambiguities and tensions at the very heart of Marx’s thought," and that it "speaks to us, like Christianity, with many voices and in accents that are at best suggestive and at worst deliberately seductive rather than precise". Eight of the articles, including that of Kamenka, can be described as being theoretical, concerned about clarification of such questions as the methodology of Marx, the place of Engels in Marxism, Marxian Ethics, Totality in Lukacs and Adorno, Sartrean Marxism, the transformation of the concept of practice from Kant via Marx to Lukacs, and the reawakened interest in the "Asiatic Mode of Production" as a stage in the evolution towards Socialism. Ten articles discuss the manifestations of Marxism in Western Europe, Russia, Italy, Zionism, the Arab world, Czechoslovakia, Poland, Latin America, and the radical student movements of the 1960s in the United States, France, and Israel. Two other articles examine the differences and reasons for differences with Marx expressed by two major commentators, Trotsky and Mao. (shrink)

Using electromagnetic levitation in combination with the oscillating drop technique and drop calorimeter method, the surface tensions and specific heats of undercooled liquid Co-10 wt% Mo, Co-26.3 wt% Mo, and Co-37.6 wt% Mo alloys were measured. The containerless state during levitation produces substantial undercoolings up to 223 K , 213 K and 110 K respectively for these three alloys. In their respective undercooling ranges, the surface tensions were determined to be 1895 m 0.31, 1932 m 0.33, and 1989 m 0.34 (...) mN m m 1 . According to the Butler equation, the surface tensions of these three Co-Mo alloys were also calculated, and the results agree well with the experimental data. The specific heats of these three alloys are determined to be 41.85, 43.75 and 44.92 J mol m 1 K m 1 . Based on the determined surface tensions and specific heats, the changes in thermodynamics functions such as enthalpy, entropy and Gibbs free energy are predicted. Furthermore, the crystal nucleation, dendrite growth and Marangoni convection of undercooled Co-Mo alloys are investigated in the light of these measured thermophysical properties. (shrink)